Bishop says peace & prosperity is everybody’s responsibility at Law Enforcers Church Service Related Items:Caribbean Hotel and Tourism Association, Caribbean Tourism Organization, Emil Lee, premier rufus ewing, Richard Sealy, tourism champion Recommended for you Beaches puts former Premier on blast about controversial pier Facebook Twitter Google+LinkedInPinterestWhatsAppProvidenciales, 26 Oct 2015 – Premier Rufus Ewing has been named a ‘Tourism Champion’ and was last week awarded for it by the Caribbean Tourism Organization, CTO. During the annual State of the Industry conference Premier Ewing received the commendation and was also featured in a panel discussion, ‘What Our Leaders Say’. The award was done in conjunction with the World Tourism Organization of the United Nations and the World Travel and Tourism Council which salute country leaders for promoting travel and tourism as ‘a driver of socioeconomic growth and development’. “State of the Industry was this year held in Curacao and the presentation was made to Hon Dr. Rufus Ewing by Barbados Tourism Minister, Richard Sealy – who is also Chairman of the CTO and President of the Caribbean Hotel and Tourism Association, Emil Lee. Facebook Twitter Google+LinkedInPinterestWhatsApp Row over Grand Turk infrastructure reaches fever pitch in Parliament
Facebook Twitter Google+LinkedInPinterestWhatsApp#TurksandCaicos, September 12, 2017 – Providenciales – Despite challenging circumstances on the islands at this time, The Royal Turks and Caicos Islands Police are continuing their task of providing service and protection to the communities of Providenciales.Detectives have arrested and charged a 35 year old male of Providenciales for Conspiracy to Rob in connection with a report made on Saturday 4th March 2017. Around 2:29am that date, police responded to the Cheshire Hall Medical where the victim told officers that upon exiting a vehicle in his hotel lobby he was approached by two masked men who shot him before making off with an undisclosed amount of money. The male is presently on remand.Yesterday morning (Monday 11th September 2017) around 1:40am, officers responded to a report of an attempted Burglary at a business establishment, a 24 year old male was apprehended and is in police custody assisting with the investigation.Around 12:29am this morning (Tuesday 12th September 2017), Tactical Unit officers responded to a firearm related offence where three persons were arrested and are assisting Police in the investigation. A firearm was also recovered and investigations are ongoing.“Officers are working hard despite some losses at their homes, we are committed to protecting the communities of the Turks and Caicos and these arrests are part of the work to tackle crime.” said Commissioner James Smith.Press Release: RTCIPF Facebook Twitter Google+LinkedInPinterestWhatsApp Related Items:
WILMINGTON, MA — Want to learn more about podcasting at Wilmington Community Television?WCTV is holding a Media Meet-Up on Wednesday, August 28, 2019 at 7pm for both beginners and advanced users. Learn how the studio is set up and get tips from other local podcasters about how they produce their programs.WCTV has a LOT of great podcasts right now. Read more about them HERE.To learn more about the event and to RSVP, contact Lisa Kapala at firstname.lastname@example.org.(NOTE: The above announcement is from Wilmington Community Television.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email email@example.com.Share this:TwitterFacebookLike this:Like Loading… RelatedNews & Notes From WCTV: Did You Know WCTV Has A Podcast Studio That YOU Can Use?In “Community”News & Notes From WCTV: WCTV Executive Director Shaun Neville Answers YOUR QuestionsIn “Community”5 Things To Do In Wilmington On Wednesday, August 28, 2019In “5 Things To Do Today”
In a statement, The North Face said: “We believe deeply in @Wikipedia’s mission and apologize for engaging in activity inconsistent with those principles. Effective immediately, we have ended the campaign and moving forward, we’ll commit to ensuring that our teams and vendors are better trained on the site policies.” Yesterday, we were disappointed to learn that @thenorthface and @LeoBurnett unethically manipulated Wikipedia. They have risked your trust in our mission for a short-lived consumer stunt. 1/ https://t.co/aIl5XEkS3z— Wikipedia (@Wikipedia) May 29, 2019 Tags Wikipedia slammed The North Face for adding product photos to the platform to boost its search ranking. NurPhoto/Getty Images Wikipedia says The North Face violated its terms by swapping photos on the site with its own in order to appear at the top of Google searches. The Wikimedia Foundation, the nonprofit that hosts Wikipedia, said Wednesday that The North Face and ad agency Leo Burnett Tailor Made “unethically manipulated” Wikipedia after removing photos on the platform and replacing them with ones featuring The North Face’s products. In an ad campaign video boasting about the strategy, the companies said they “hacked the results” so that The North Face appears at the top of Google whenever someone searches for an adventure. They said they paid “absolutely nothing just by collaborating with Wikipedia.” The Wikimedia Foundation denied involvement in the campaign. “What they did was akin to defacing public property,” the Wikimedia Foundation said in a post. “When The North Face exploits the trust you have in Wikipedia to sell you more clothes, you should be angry. Adding content that is solely for commercial promotion goes directly against the policies, purpose and mission of Wikipedia to provide neutral, fact-based knowledge to the world.”Volunteers with the Wikimedia Foundation took down The North Face’s images or cropped out the logos and are ensuring the affected articles “meet Wikipedia’s standards of neutrality and reliable sourcing,” the nonprofit said.Wikipedia tweeted about the incident, saying: “Yesterday, we were disappointed to learn that @thenorthface and @LeoBurnett unethically manipulated Wikipedia. They have risked your trust in our mission for a short-lived consumer stunt.” Google 0 Share your voice 1:25 Internet Now playing: Watch this: Post a comment Google’s head of advertising calls for privacy, but not…
A young man was hacked to death allegedly by his gambling rivals at Shamirpur village in Karnaphuli upazila of Chattogram on Wednesday night, reports UNB.The deceased is Mamun Al-Rashid Sagor, 27, son of Abu Taher of the village.Rafiqul Islam, officer-in-charge of Karnaphuli police station, said two groups locked into an altercation over gambling and drugs.At one stage, opponent group hacked Mamun, leaving him dead on the spot. Police started drive to arrest the accused who were involved in this connection, the OC added.
File photoSome 1,096 people suffering from dengue fever were hospitalised across the country in the last 24 hours till 8am on Monday, showing a gradual rise in the number of such patients over the last several days.However, there was no death during the period as per the official record, reports UNB.According to the Directorate General of Health Services (DGHS), the number of dengue patients was 824 on Sunday while 683 on Saturday, 603 on Friday and 547 on Thursday.The dengue has now spread to 50 districts of the country.Currently, 3,847 dengue patients are being treated in hospitals across the country.From 1 January till the date, there have been 13,637 confirmed dengue cases.Eight of them have died as per the government’s count although the unofficial death toll is much higher.Meanwhile, the health and family welfare ministry has set up a monitoring cell to receive allegations about charging extra than the government-fixed Tk 500 for dengue test.
By Associated PressHundreds of people marched to honor the memory of a 4-year-old Houston girl who was missing for several weeks before her remains were found last month in Arkansas.Family members of Maleah Davis as well as area residents walked through downtown Houston on Sunday.Hundreds gathered in downtown Houston on June 9 to honor the legacy of 4-year-old Maleah Davis, who was missing for weeks, before her remains were later found in Arkansas. (AP Photo)After the march, people gathered at City Hall. Craig Davis, Maleah’s father, spoke briefly, thanking the crowd for their support and saying the march meant so much to his family.The city of Houston declared Sunday as “Maleah Davis Day.” Later in the evening, officials lit up City Hall in pink lights, which was the girl’s favorite color.Derion Vence, the ex-fiance of Maleah’s mother, is jailed on a tampering with evidence charge in the case. Authorities say additional charges could be filed.
Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. More information: Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces, PNAS November 26, 2013 vol. 110 no. 48 19396-19401, doi:10.1073/pnas.1311761110 Moreover, Kaar continues, monitoring molecule-by-molecule structure changes in organophosphorus hydrolase had its own challenges related to eliminating mislabeled protein molecules – that is, molecules with other than one donor and one acceptor fluorophore – from analysis. “We met this challenge by creating and implementing filters during data analysis that separated signals from properly labeled and mislabeled species.”Kaar points out that using SM-FRET tracking had its own issue. For one, it required high-throughput tracking algorithms (developed by co-authors Kastantin and Schwartz) critical to monitor changes in FRET signals for large numbers of molecules, which in turn was essential to identifying protein structure changes accurately (that is, with high statistical confidence). He points out that SM-FRET also required prior knowledge of the crystal structure of OPH, which was needed to make the FRET signal indicative of quantitative changes in protein conformation.The study’s results suggest that surfaces may act as a source of unfolded (that is, aggregation-prone) protein back into solution – but validating this implication faces the challenge of identifying the conformation of protein molecules immediately before desorption from the surface. “The question of whether the unfolded proteins induced aggregation in solution after desorption remains to be fully understood,” Kaar explains. “Fully understanding if this is actually the case requires further analysis of protein in solution in the presence of the surface.”The team leveraged two key innovations to address these research challenges – the implementation of site-specific labeling methods, and high-throughput tracking algorithms with SM-FRET. “Combining these methods enabled the decoupling of surface-induced conformational changes from protein adsorption and desorption events,” Kaar notes. “By decoupling such phenomenon, this approach allowed us to overcome the limitations of conventional surface characterization methods.”The research also shows that SM-FRET permits a unique level of understanding of the ways in which surface chemistry influences molecular conformation and, in turn, function. “By observing molecular-level changes in protein structure in isolation from competing surface dynamics, it’s easier to make a direct connection between surface chemistry and conformation,” Kaar points out. “Therefore, it is more straightforward to see the effects of surface chemistry and can lead to new ideas for how to improve chemistry for a given application.Another important finding is that the new method will enable the creation of surfaces and modifications with improved biocompatibility by uncovering the connection between surface properties and protein unfolding. “This connection is critical to inspiring and developing surfaces and modifications that meld with the biological world,” Kaar explains. “For example, with this understanding, we can begin to design surfaces that promote protein folding and therefore favorable responses from cells present in the surrounding milieu. In this example, the folded state of the protein may display certain biological signals to cells that thwart unwanted inflammatory or harmful reactions while instructing cells to respond in ways that may facilitate proliferation, differentiation or even wound healing in vivo.”Kaar tells Phys.org that future experiments are aimed at determining if the observed effects of fused silica on organophosphorus hydrolase are general or specific to this combination of surface and protein. “We plan to address this question by probing how fused silica and surfaces with other properties impact the folding of other proteins. We’re also interested in expanding our methods to understand how surface effects on conformation impact the binding of a third protein species. Understanding this impact is critical to, for example, enumerating how cells respond to biological cues on surfaces in physiological environments.” Other innovations that the researchers may develop, Kaar adds, include more sophisticated labeling to minimize SM-FRET protein mislabeling on surfaces, as well as labeling and detection schemes to enable multiple molecular events, including unfolding and binding, to be monitored simultaneously.”Given that the interaction of proteins and surfaces are relevant in virtually all areas of biotechnology,” Kaar notes, “many other areas of research – for example, tissue engineering and regenerative medicine, biosensing, biocatalysis, and pharmaceutical protein formulation – may benefit from exploiting our approach.” (Phys.org) —Proteins accomplish something rather amazing: A protein can have many functions, with a given function being determined by the way they fold into a specific three-dimensional geometry, or conformations. Moreover, the structural transitions form one conformation to another is reversible. However, while these dynamics affect protein conformation and therefore function, and so are critical to a wide range of areas, methods for understanding how proteins behave near surfaces, which is complicated by protein and surface heterogeneities, has remained elusive. Recently, however, scientists at University of Colorado utilized a method known as Single-Molecule Förster Resonance Energy Transfer (SM-FRET) tracking to monitor dynamic changes in protein structure and interfacial behavior on surfaces by single-molecule Förster resonance energy transfer, allowing them to explicate changes in protein structure at the single-molecule level. (SM-FRET describes energy transfer between two chromophores – molecular components that determine its color.) In addition, the researchers state that their approach is suitable for studying virtually any protein, thereby providing a framework for developing surfaces and surface modifications with improved biocompatibility. Journal information: Proceedings of the National Academy of Sciences © 2013 Phys.org. All rights reserved. Citation: Two for the price of one: Single-molecule microscopy simultaneously monitors protein structure and function (2013, December 4) retrieved 18 August 2019 from https://phys.org/news/2013-12-price-single-molecule-microscopy-simultaneously-protein.html Protein surfaces defects act as drug targets Structure of OPH showing the position of site-specific donor and acceptor labeling. OPH is a homodimer (C2 symmetry) that consists of two (α/β)8 monomers. The position K175, which was replaced with AzF in monomers A and B of OPH, is highlighted (yellow). Credit: Copyright © PNAS, doi:10.1073/pnas.1311761110 Prof. Joel L. Kaar discussed the paper he and his co-authors, Dr. Sean Yu McLoughlin, Prof. Mark Kastantin and Prof. Daniel K. Schwartz, recently published in Proceedings of the National Academy of Sciences. “The primary challenges in devising our approach to characterizing changes in protein structure were implementing a site-specific labeling method, which enabled single-molecule resolution, as well as a method to only image molecules at the solution-surface interface,” Kaar tells Phys.org. The scientists overcame the former challenge by incorporating unnatural amino acids – that is, those not among the 20 so-called standard amino acids – with unique functional groups for labeling with fluorophores (chemical compounds that can re-emit light upon light excitation); the latter, by using total internal reflection fluorescence microscopy, which only excites molecules in the near-surface environment, thereby minimizing the background fluorescence of molecules free in solution. “Although site-specific labeling methods have been used to monitor changes in protein conformation mainly in bulk solution, such techniques have not previously been exploited to study freely diffusible protein molecules at interfaces,” Kaar adds. As such, the researchers are the first to apply site-specific labeling methods to study protein-surface interactions,”The major challenge associated with incorporating unnatural amino acids for labeling was related to the optimization of protein expression,” Kaar continues. Specifically, he explains, the expression of the enzyme organophosphorus hydrolase (OPH) – which is notoriously difficult to make in large quantities due to inclusion body formation – with the unnatural amino acid p-azido-L-Phe (AzF) had to be optimized to efficiently incorporate p-azido-L-Phe. (Inclusion body formation refers to the intracellular aggregation of partially folded expressed proteins,) “This process required modification of expression conditions,” he adds, “in which bacteria with modified genetic machinery were grown to enable production of soluble enzyme for single-molecule experiments.”
It is essential for a bride to add a healthy haircare routine and pick up the right lingerie for that perfect night and pamper her own self. People assume that since most of the wedding outfits come with inner padding, there’s no need to invest in good lingerie. But this assumption is wrong and it will eventually lead up to your wedding lingerie dilemma. Experts share their thought on essential things to add to your bridal kit. One of the most common problems faced by the thin and petite ones is trying to get that extra oomph. In this case, the push bra paired with a lace bikini is the best option. The brassiere’s underwire and push up padding will give you the lift you need which will leave you feeling like a million bucks. Also Read – Add new books to your shelfTea tree oil awakens, refreshes and re-energises your skin; and strengthens and regenerates you hair. One can also opt for products with Tea Tree oil that offers care, styling and body products to renew the scalp and hair, and revitalise your charm. If you are voluptuous with large assets and think you can’t find anything to suit your style well you’re wrong. The lace unlined bra and thong set is what you need to show off your curves. Turn up the heat with the lace back thong along with the unlined bra which does a fabulous job in highlighting your natural shape. If you are long waisted i.e the ones whose upper body are longer than the lower half. The satin low back nightdress is perfect to show off your enviable waist. Plus, the plunge neck and low back will leave you looking sexy while adding a hint of sophistication. Ease your haircare routine by taking essential care in the right manner for elegant, salon quality treatment like spa, to have a relaxing time.
11Apr LaFave outraged by proposed registration fees for kayaks, canoes Categories: LaFave News Legislator opposes increased taxation for Michigan outdoor enthusiastsState Rep. Beau LaFave today expressed his objection to a recent proposal by the Michigan State Waterways Commission that would require all kayaks, canoes and paddle boards to be registered with the state.LaFave said people should not be penalized for supporting Michigan’s tourism industry and enjoying the state’s natural resources, especially considering participation in paddle sports increases at a rate of 7 percent each year in Michigan.“This is one of the silliest things I’ve heard in my time as representative,” said LaFave, of Iron Mountain. “What’s next? Will we have to register our water skis, our boogie boards, our wetsuits?”Though the commission claims the cost would be about $10 annually per watercraft, LaFave still has concerns.“They’re not thinking about those who have many paddle watercrafts,” he said, “the burden could add up to hundreds of dollars annually for moms and dads who have kayaks or paddle boards for each member of their family, not to mention the absurd costs for summer camps and rental companies.”Thus far, the commission’s proposal hasn’t gained any momentum, as no legislators have pursued the matter with legislation, but LaFave said he’s certain he won’t support it if put in a position to vote on it.###