Gio Simeone is still going to work hard in order to become an Atletico Madrid player someday, but he wants to avoid nepotism in Argentina.The sentiment that awakens Atletico Madrid in some players is simply inevitable sometimes, Argentina international Gio Simeone is the best example of this because he doesn’t care that his father doesn’t want to coach him there for now.Any professional football player who has the characteristic fighting spirit that any Atletico Madrid player historically has, would have the dream of someday playing for the Colchoneros regardless of the circumstances.The oldest of Diego Simeone’s sons heard what his father had to say about the possibility of coaching him someday in the Spanish club, but he doesn’t seem to care about the response.Giovanni Simeone has the dream of playing for Atletico Madrid in the future because he is a true fan of the institution, he doesn’t care if his father is still the manager of the squad when he reaches a level that is good enough for the club to pursue him.The trades that this striker has are very similar to what the club has always wanted in their best players, which makes the possibility even greater for him in the near future.All Gio has to do is keep working hard, and hope that he can get that call soon.”ME ENCANTARÍA VER A MI PAPÁ ACÁ””SIEMPRE ME DICE QUE NO ES EL MOMENTO”#CentralFOX | Giovanni Simeone opinó sobre la situación de su padre como posible entrenador de la Selección Argentina. pic.twitter.com/90fIWMnYzm— FOX Sports Argentina (@FOXSportsArg) October 14, 2018The eldest of Simeone’s kids granted an interview with Fox Sports, where he discussed his father again and the upcoming friendly match between Argentina and Brazil on Tuesday.“Atletico Madrid is something that has been practically taught to us, it’s inside me and my dad, but I never had the chance of being coached by him.”“My old man has never talked to me about this possibility. The fact that people keep talking about this means that I’m still growing.”“Playing for Atletico Madrid someday would be truly amazing for me, an incredible achievement. As an Argentina player, I would love to see my dad as the manager here, but he keeps saying that it’s not the right time yet.”La Liga Betting: Match-day 4 Stuart Heath – September 14, 2019 Despite it being very early into La Liga season, both Barcelona and Real Madrid have had unprecedented starts to their campaigns. With this in…“Every single person from Argentina would like having him here because he is a great manager, one of the best in the world,” said Gio during the interview.Gran partido de todo el equipo! #VamosArgentina 🇦🇷👊🏼⚽️ pic.twitter.com/gAmMBkBE4Z— Giovanni Simeone (@simeonegiovanni) October 11, 2018Giovanni is getting ready to represent his country on his very first Classic match between Argentina and Brazil, he didn’t play much against Saudi Arabia last week but he could get more minutes for this match and he hopes he can live up to the family name.Getting a great participation in matches like this one, are what can assure him a permanent spot in the squad, something that he aims for before his father gets the job as the manager.Gio Simeone is very aware of nepotism, which is something that both he and his father are always trying to avoid.“If I keep getting called-up and I keep having minutes with this group, the possibility of us crossing paths here becomes greater. Things would be obviously different if I was never called-up and he calls me right after he gets the job,” he added.“What I aim for is to win a spot here before he gets the position, so I’m fighting against the clock. I’ve been preparing for this possibility this year, but I honestly didn’t expect a call-up this soon.”“My grandfather gave me a very useful advice. He told me that one minute in the national team is the equivalent of sixty minutes in a club. This means that I will give my very best in every minute I get a chance to play for Argentina.”“I’ve been in Europe for only two years, so if I already had this chance, is because I can still give a lot more than even I expected,” he concluded.Preparando el partido de mañana 💪🏼⚽️🇦🇷 #VamosArgentina pic.twitter.com/t8qKVDtbUC— Giovanni Simeone (@simeonegiovanni) October 10, 2018How well do you think Giovanni Simeone would do with his father as the manager? Please share your opinion in the comment section down below.
Mats Seuntjens couldn’t believe his eyes as his brilliant chip bounced away from the goal just as he begun to celebrateThe Dutch midfielder chipped the ball from the edge of the penalty area on the right side to potentially hand AZ Alkmaar an unlikely goal in their KNVB Cup tie with PEC Zwolle.The ball went past the helpless Zwolle goalkeeper and appeared to be heading straight into the goal only for it to then take a backspin off the goal line.This left a flabbergasted Seuntjens crutching his hips wondering what happened.“Unbelievable. Unfortunately, there was just too much effect on the ball,” said Seuntjens afterwards, via talkSPORT.Europa League draw groups K and L George Patchias – August 30, 2019 Europa League groups K and L were kind to Wolves and United, weren’t they?UEFA.com confirmed that Europa League group K consists of, Besiktas, Braga,…“Really too bad, because it was a great ball, even if I say so myself. ”Although AZ did still win 5-0 anyway and progressed through to the quarter-finals of the KNVB Cup.Here’s Ralf Seuntjes of AZ scoring a delightful lob over the… hang on, nope.pic.twitter.com/T21KEw866O— GoalScorer Challenge (@GoalscorerC) December 19, 2018
Facebook0TwitterEmailPrintFriendly分享The Soldotna Police Department was notified on Friday of a student making a ‘joke’ of school violence in a classroom at Soldotna Prep. According to Schmidt out of an over-abundance of caution he reported the incident to the Soldotna Police Department. In an email to parents from Curtis Schmidt, Principal at Soldotna Prep, around 1:40pm, a student walked into their classroom, and jokingly made a statement about school violence. The situation was immediately diffused when the classroom aide escorted the student to the principal’s office, was questioned, and policy is being followed regarding student discipline. Parents were sent an email from Schmidt late on Friday. Schmidt: “Our school has zero tolerance for any jokes or comments that create concern for the safety of our staff and students. The school remained open all day on a normal schedule.”
WILMINGTON, MA — Want to learn more about podcasting at Wilmington Community Television?WCTV is holding a Media Meet-Up on Wednesday, August 28, 2019 at 7pm for both beginners and advanced users. Learn how the studio is set up and get tips from other local podcasters about how they produce their programs.WCTV has a LOT of great podcasts right now. Read more about them HERE.To learn more about the event and to RSVP, contact Lisa Kapala at firstname.lastname@example.org.(NOTE: The above announcement is from Wilmington Community Television.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email email@example.com.Share this:TwitterFacebookLike this:Like Loading… RelatedNews & Notes From WCTV: Did You Know WCTV Has A Podcast Studio That YOU Can Use?In “Community”News & Notes From WCTV: WCTV Executive Director Shaun Neville Answers YOUR QuestionsIn “Community”5 Things To Do In Wilmington On Wednesday, August 28, 2019In “5 Things To Do Today”
In a statement, The North Face said: “We believe deeply in @Wikipedia’s mission and apologize for engaging in activity inconsistent with those principles. Effective immediately, we have ended the campaign and moving forward, we’ll commit to ensuring that our teams and vendors are better trained on the site policies.” Yesterday, we were disappointed to learn that @thenorthface and @LeoBurnett unethically manipulated Wikipedia. They have risked your trust in our mission for a short-lived consumer stunt. 1/ https://t.co/aIl5XEkS3z— Wikipedia (@Wikipedia) May 29, 2019 Tags Wikipedia slammed The North Face for adding product photos to the platform to boost its search ranking. NurPhoto/Getty Images Wikipedia says The North Face violated its terms by swapping photos on the site with its own in order to appear at the top of Google searches. The Wikimedia Foundation, the nonprofit that hosts Wikipedia, said Wednesday that The North Face and ad agency Leo Burnett Tailor Made “unethically manipulated” Wikipedia after removing photos on the platform and replacing them with ones featuring The North Face’s products. In an ad campaign video boasting about the strategy, the companies said they “hacked the results” so that The North Face appears at the top of Google whenever someone searches for an adventure. They said they paid “absolutely nothing just by collaborating with Wikipedia.” The Wikimedia Foundation denied involvement in the campaign. “What they did was akin to defacing public property,” the Wikimedia Foundation said in a post. “When The North Face exploits the trust you have in Wikipedia to sell you more clothes, you should be angry. Adding content that is solely for commercial promotion goes directly against the policies, purpose and mission of Wikipedia to provide neutral, fact-based knowledge to the world.”Volunteers with the Wikimedia Foundation took down The North Face’s images or cropped out the logos and are ensuring the affected articles “meet Wikipedia’s standards of neutrality and reliable sourcing,” the nonprofit said.Wikipedia tweeted about the incident, saying: “Yesterday, we were disappointed to learn that @thenorthface and @LeoBurnett unethically manipulated Wikipedia. They have risked your trust in our mission for a short-lived consumer stunt.” Google 0 Share your voice 1:25 Internet Now playing: Watch this: Post a comment Google’s head of advertising calls for privacy, but not…
X Share Al OrtizHouston Mayor Sylvester Turner (center) announces the results of an investigation by the Houston Police Department about the sale of Kush on the city. Houston Police DepartmentKush is typically packaged in shiny and visually appealing bags. Houston Police DepartmentRafeeq Panjvany is one of the two men HPD arrested as a result of this investigation. Houston Police DepartmentNaushad Pradhan is the other man HPD arrested as a result of this investigation.Houston Mayor Sylvester Turner announced Monday the results of an investigation about the sale of synthetic marijuana, which has become an ongoing mission for the Houston Police Department, HPD.Accompanied by HPD’s acting chief Martha Montalvo and Harris County District Attorney Devon Anderson, Turner held a press conference at HPD’s headquarters to inform about the arrest of two men, Naushad Ramzan-AliPradhan (58 years-old) and Rafeeq Panjvany (54 years-old), who have been charged with delivery of synthetic cannabinoids in the 208th State District Court.The investigation began in early July through an anonymous tip and culminated with the arrest of the two men on September 14th.HPD recovered more than 4,000 packages of Kush—the common name of the drug— with a street value of over 400,000 dollars.HPD Sergeant Marsha Todd said they are making progress in the fight against Kush and detailed that “the Narcotics Division right now has made approximately 46 total arrests from various stores and other locations since our Kush initiative began in late June.”“We’ve seized hundreds of pounds of Kush, along with various assets of the proceeds from the sale of these synthetic drugs,” Todd added.Kush has been in the news recently after an incident at Hermann Park, when about ten individuals were taken to local hospitals for overdoses of synthetic marijuana.Turner said Kush has become a national epidemic and Houston is no stranger to its effects.“In the four months since it was created,” the Mayor noted “HPD’s public intoxication team has responded to approximately 600 calls, resulting in nearly 500 individuals taken off the streets and admitted to the City’s sobering centers.”HPD officials emphasize they are trying to go after the suppliers to have a long term effect in their fight against this drug.The public can submit anonymous tips through the website www.stopdrugshouston.org. 00:00 /01:19 Listen To embed this piece of audio in your site, please use this code:
By Associated PressHundreds of people marched to honor the memory of a 4-year-old Houston girl who was missing for several weeks before her remains were found last month in Arkansas.Family members of Maleah Davis as well as area residents walked through downtown Houston on Sunday.Hundreds gathered in downtown Houston on June 9 to honor the legacy of 4-year-old Maleah Davis, who was missing for weeks, before her remains were later found in Arkansas. (AP Photo)After the march, people gathered at City Hall. Craig Davis, Maleah’s father, spoke briefly, thanking the crowd for their support and saying the march meant so much to his family.The city of Houston declared Sunday as “Maleah Davis Day.” Later in the evening, officials lit up City Hall in pink lights, which was the girl’s favorite color.Derion Vence, the ex-fiance of Maleah’s mother, is jailed on a tampering with evidence charge in the case. Authorities say additional charges could be filed.
The University of Louisville’s Danielle Watson was named ACC Co-Pitcher of the Week for the period ending April 7. This marks the sophomore’s first conference weekly honor.Watson posted a 0.00 ERA on the week surrendering only one unearned run and two hits for a .053 opp. batting average and striking out 13 in 12.0 innings of work.The Osceola, Ind., native posted a complete game one-hit shutout and struck out seven in Louisville’s 2-0 upset of No. 3/3 Florida State which entered the weekend ranked second in the nation in batting average and third in home runs. In that game, she retired 10 Seminole batters at one point and 16 of the last 18 to end the game and halt the Seminoles’ 10-game win streak. Watson also pitched 6.1 innings in a 10-9 win against Florida State on Monday.In Louisville’s midweek 7-2 win at Western Kentucky, Watson struck out three as she retired all six batters she faced in the final two innings. She also connected on her first career home run in that game.Watson now leads the team with 112 strikeouts in 106.2 innings and a .213 opponent batting average.The Cardinals (25-14, 6-6 ACC) will travel to Boston College for a three-game ACC series this weekend. Print Friendly Version Story Links
Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. More information: Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces, PNAS November 26, 2013 vol. 110 no. 48 19396-19401, doi:10.1073/pnas.1311761110 Moreover, Kaar continues, monitoring molecule-by-molecule structure changes in organophosphorus hydrolase had its own challenges related to eliminating mislabeled protein molecules – that is, molecules with other than one donor and one acceptor fluorophore – from analysis. “We met this challenge by creating and implementing filters during data analysis that separated signals from properly labeled and mislabeled species.”Kaar points out that using SM-FRET tracking had its own issue. For one, it required high-throughput tracking algorithms (developed by co-authors Kastantin and Schwartz) critical to monitor changes in FRET signals for large numbers of molecules, which in turn was essential to identifying protein structure changes accurately (that is, with high statistical confidence). He points out that SM-FRET also required prior knowledge of the crystal structure of OPH, which was needed to make the FRET signal indicative of quantitative changes in protein conformation.The study’s results suggest that surfaces may act as a source of unfolded (that is, aggregation-prone) protein back into solution – but validating this implication faces the challenge of identifying the conformation of protein molecules immediately before desorption from the surface. “The question of whether the unfolded proteins induced aggregation in solution after desorption remains to be fully understood,” Kaar explains. “Fully understanding if this is actually the case requires further analysis of protein in solution in the presence of the surface.”The team leveraged two key innovations to address these research challenges – the implementation of site-specific labeling methods, and high-throughput tracking algorithms with SM-FRET. “Combining these methods enabled the decoupling of surface-induced conformational changes from protein adsorption and desorption events,” Kaar notes. “By decoupling such phenomenon, this approach allowed us to overcome the limitations of conventional surface characterization methods.”The research also shows that SM-FRET permits a unique level of understanding of the ways in which surface chemistry influences molecular conformation and, in turn, function. “By observing molecular-level changes in protein structure in isolation from competing surface dynamics, it’s easier to make a direct connection between surface chemistry and conformation,” Kaar points out. “Therefore, it is more straightforward to see the effects of surface chemistry and can lead to new ideas for how to improve chemistry for a given application.Another important finding is that the new method will enable the creation of surfaces and modifications with improved biocompatibility by uncovering the connection between surface properties and protein unfolding. “This connection is critical to inspiring and developing surfaces and modifications that meld with the biological world,” Kaar explains. “For example, with this understanding, we can begin to design surfaces that promote protein folding and therefore favorable responses from cells present in the surrounding milieu. In this example, the folded state of the protein may display certain biological signals to cells that thwart unwanted inflammatory or harmful reactions while instructing cells to respond in ways that may facilitate proliferation, differentiation or even wound healing in vivo.”Kaar tells Phys.org that future experiments are aimed at determining if the observed effects of fused silica on organophosphorus hydrolase are general or specific to this combination of surface and protein. “We plan to address this question by probing how fused silica and surfaces with other properties impact the folding of other proteins. We’re also interested in expanding our methods to understand how surface effects on conformation impact the binding of a third protein species. Understanding this impact is critical to, for example, enumerating how cells respond to biological cues on surfaces in physiological environments.” Other innovations that the researchers may develop, Kaar adds, include more sophisticated labeling to minimize SM-FRET protein mislabeling on surfaces, as well as labeling and detection schemes to enable multiple molecular events, including unfolding and binding, to be monitored simultaneously.”Given that the interaction of proteins and surfaces are relevant in virtually all areas of biotechnology,” Kaar notes, “many other areas of research – for example, tissue engineering and regenerative medicine, biosensing, biocatalysis, and pharmaceutical protein formulation – may benefit from exploiting our approach.” (Phys.org) —Proteins accomplish something rather amazing: A protein can have many functions, with a given function being determined by the way they fold into a specific three-dimensional geometry, or conformations. Moreover, the structural transitions form one conformation to another is reversible. However, while these dynamics affect protein conformation and therefore function, and so are critical to a wide range of areas, methods for understanding how proteins behave near surfaces, which is complicated by protein and surface heterogeneities, has remained elusive. Recently, however, scientists at University of Colorado utilized a method known as Single-Molecule Förster Resonance Energy Transfer (SM-FRET) tracking to monitor dynamic changes in protein structure and interfacial behavior on surfaces by single-molecule Förster resonance energy transfer, allowing them to explicate changes in protein structure at the single-molecule level. (SM-FRET describes energy transfer between two chromophores – molecular components that determine its color.) In addition, the researchers state that their approach is suitable for studying virtually any protein, thereby providing a framework for developing surfaces and surface modifications with improved biocompatibility. Journal information: Proceedings of the National Academy of Sciences © 2013 Phys.org. All rights reserved. Citation: Two for the price of one: Single-molecule microscopy simultaneously monitors protein structure and function (2013, December 4) retrieved 18 August 2019 from https://phys.org/news/2013-12-price-single-molecule-microscopy-simultaneously-protein.html Protein surfaces defects act as drug targets Structure of OPH showing the position of site-specific donor and acceptor labeling. OPH is a homodimer (C2 symmetry) that consists of two (α/β)8 monomers. The position K175, which was replaced with AzF in monomers A and B of OPH, is highlighted (yellow). Credit: Copyright © PNAS, doi:10.1073/pnas.1311761110 Prof. Joel L. Kaar discussed the paper he and his co-authors, Dr. Sean Yu McLoughlin, Prof. Mark Kastantin and Prof. Daniel K. Schwartz, recently published in Proceedings of the National Academy of Sciences. “The primary challenges in devising our approach to characterizing changes in protein structure were implementing a site-specific labeling method, which enabled single-molecule resolution, as well as a method to only image molecules at the solution-surface interface,” Kaar tells Phys.org. The scientists overcame the former challenge by incorporating unnatural amino acids – that is, those not among the 20 so-called standard amino acids – with unique functional groups for labeling with fluorophores (chemical compounds that can re-emit light upon light excitation); the latter, by using total internal reflection fluorescence microscopy, which only excites molecules in the near-surface environment, thereby minimizing the background fluorescence of molecules free in solution. “Although site-specific labeling methods have been used to monitor changes in protein conformation mainly in bulk solution, such techniques have not previously been exploited to study freely diffusible protein molecules at interfaces,” Kaar adds. As such, the researchers are the first to apply site-specific labeling methods to study protein-surface interactions,”The major challenge associated with incorporating unnatural amino acids for labeling was related to the optimization of protein expression,” Kaar continues. Specifically, he explains, the expression of the enzyme organophosphorus hydrolase (OPH) – which is notoriously difficult to make in large quantities due to inclusion body formation – with the unnatural amino acid p-azido-L-Phe (AzF) had to be optimized to efficiently incorporate p-azido-L-Phe. (Inclusion body formation refers to the intracellular aggregation of partially folded expressed proteins,) “This process required modification of expression conditions,” he adds, “in which bacteria with modified genetic machinery were grown to enable production of soluble enzyme for single-molecule experiments.”
The festival season in Kolkata is going to see a crop of new detective films in Bengali- a Byomkesh Bakshi thriller and a Feluda flick. Double Feluda is an upcoming thriller directed by acclaimed director Sandip Ray, son of the iconic film director Satyajit Ray.Occasion? Feluda was created by the acclaimed Oscar-winning director 50 years ago and son Sandip Ray’s latest Feluda film is based on this popular Bengali sleuth’s many exploits. This film also marks the comeback of veteran actor Sabyasachi Chakraborty as Feluda (after Royal Bengal Rahasya in 2011) and Saheb Bhattacharya, as his assistant Topshe. Also Read – Add new books to your shelfThe film is scheduled to be released in mid-December. Two stories, ‘Samaddarer Chabi’ and ‘Golokdham Rahasya’ are going to be packed in this thriller. The popular character Jatayu of other Feluda stories and films will probably be missing from these films. Just a year after hitting the silver screen with the popular Har Har Byomkesh, director Arindam Sil will be back once again with a revamped Byomkesh Bakshi – Byomkesh Pawrbo- starring Abir Chatterjee in the lead role. Also Read – Over 2 hours screen time daily will make your kids impulsiveBased on Sharadindu Bandhopadhyay’s classic series, the cerebral Bengali sleuth Byomkesh Bakshi will be revealing his daring side as he pulls off some daredevil stunts in the Dooars forest where the story is set. A new riveting case sees Bakshi playing investigator in Santagola which has become a hub of the illegal weapons trade. The Bengal Government requests the sleuth to track down the culprits but as soon as Bakshi starts his investigation, he zeroes in on few well-known people of this city, who turn out to be possible suspects. However, he realises that these men are powerful and he will never be able to search their warehouses and mills. In the meantime, a demented man named Amrito claims that he has seen a strange, horse-riding figure roaming around the jungle. Amrito is sent to the jungle to observe this phenomenon. The very next morning, his bullet ridden dead body is found inside the jungle. As Byomkesh delves deep into the case, a lot of well-respected men gets unmasked.Director Arindam Sil explains why going back to this particular series is important to him, “Byomkesh Bakshi is one character in Bengali literature whose depth lies in the multiple layers of his persona. Though he is a deep thinker, a ‘Satyanweshi’ yet his unflinching courage, righteous nature and dauntless spirit make him stand apart from the rest”. The key attractions of the film, says Sil, are the beauty of the Dooars forest where the film has been extensively shot, the backdrop of the Tebhaga movement of 1948 and superb acting by an eclectic cast, including Abir Chatterjee (Byomkesh), Sohini Sarkar, Ritwick Chakraborty, Rajatava Dutta, June Maliah, Sayantika Banerjee, Kaushik Sen and other accomplished character actors of Bengali cinema. Releasing on December 12, the film is bound to face off with the Feluda release but Sil is confident about his success, “I am my own competition. Also, if Sandip Ray is liberal enough to give me rights to making another Feluda film, I’m game”. The film has been produced by Shree Venkatesh Films and Surinder Films while Sandip Ray’s Double Feluda has been produced by Eros International.